S ize - dependent free solution DNA electrophoresis in structured microfluidic systems
نویسندگان
چکیده
A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (, 3 mm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that l-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress. 2003 Elsevier Science B.V. All rights reserved.
منابع مشابه
Sequence Dependent Mobility of Short Oligonucleotides in Free- Solution Electrophoresis
INTRODUCTION Since completion of the human genome project, many additional organisms have been sequenced and ~1.8 million human genetic Single Nucleotide Polymorphisms (SNPs) have been identified and documented by the SNP consortium, http://snp.cshl.org. While the technology workhorses for these efforts have been gel-based and capillary electrophoresis DNA sequencers, there is increased interes...
متن کاملConcurrent DNA Preconcentration and Separation in Bipolar Electrode-Based Microfluidic Device.
This paper presents a bipolar electrode (BPE) device in a microfluidic dual-channel design for concurrent preconcentration and separation of composite DNA containing samples. The novelty of the present effort relies on the combination of BPE-induced ion concentration polarization (ICP) and end-labeled free-solution electrophoresis (ELFSE). The ion concentration polarization effect arising from ...
متن کاملSingle cell manipulation, analytics, and label-free protein detection in microfluidic devices for systems nanobiology.
Single cell analytics for proteomic analysis is considered a key method in the framework of systems nanobiology which allows a novel proteomics without being subjected to ensemble-averaging, cell-cycle, or cell-population effects. We are currently developing a single cell analytical method for protein fingerprinting combining a structured microfluidic device with latest optical laser technology...
متن کاملFault Tolerant DNA Computing Based on Digital Microfluidic Biochips
Historically, DNA molecules have been known as the building blocks of life, later on in 1994, Leonard Adelman introduced a technique to utilize DNA molecules for a new kind of computation. According to the massive parallelism, huge storage capacity and the ability of using the DNA molecules inside the living tissue, this type of computation is applied in many application areas such as me...
متن کاملResolution of multiple ssDNA structures in free solution electrophoresis.
By using high concentrations of buffer, electroosmotic flow within uncoated channels of a microfluidic chip was minimized, allowing the free solution electrophoretic separation of DNA. More importantly, because of the ability to efficiently dissipate heat within these channels, field strengths as high as 600 V/cm could be applied with minimal Joule heating (<2 degrees C). As a result of the hig...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003